Exploring global gecko conservation in zoos, our study utilizes ZIMS and ZTL databases to assess the distribution patterns of threatened Gekkota species. Only 9.3% of recognized gecko species are kept, with 20.4% classified as threatened. While European, North American, and Australian zoos house most species, Oceania prioritizes native geckos. Breeding success is observed in 38.0% of zoo-kept threatened species, often in one institution per species. Despite being the most species-rich lizard group, Gekkota representation in zoos is limited. Collaborative efforts with experts and conservation programs can enhance zoo contributions to global gecko conservation.