Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), poses a global threat to wheat production. Due to the limited number of resistance genes in wheat and the pathogen’s high variability, resistance can rapidly diminish. This study assessed powdery mildew resistance in 332 wheat germplasms at seedling and adult stages. Seven, four, and two accessions showed resistance to specific Bgt races, while 34 were resistant at the adult stage. Molecular analysis revealed the presence of various Pm genes, offering valuable insights for breeding durable resistance in wheat varieties and expanding genetic diversity.